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NEN Life Science micromax human cdna system i—direct kit
Micromax Human Cdna System I—Direct Kit, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cdna+system+i+direct+kit/micromax+direct+cdna+microarray+system/us08034790-656-14-8
Average 90 stars, based on 1 article reviews
micromax human cdna system i—direct kit - by Bioz Stars, 2026-09
90/100 stars

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Cell Culture:

Article Title: Stimulating parathyroid cell proliferation and PTH release with phosphate in organ cultures obtained from patients with primary and secondary hyperparathyroidism for a prolonged period.
Article Snippet: The pathogenesis of primary hyperparathyroidism (I -HPT) and secondary hyperparathyroidism (II -HPT) remains to be elucidated.. To characterize their pathophysiology, we investigated the effects of calcium and phosphate on cell proliferation and PTH release in an organ culture of parathyroid tissues.. Dissected parathyroid tissues obtained from patients with I -HPT (adenoma) or II -HPT (nodular hyperplasia) were precultured on a collagen-coated membrane for 1–4 week.

Article Title: Genes regulated by thyrotropin and iodide in cultured human thyroid follicles: analysis by cDNA microarray.
Article Snippet: Thyrotropin (TSH) regulates a number of genes in thyrocytes, leading to iodide uptake, de novo synthesis and release of thyroid hormones, and cell proliferation, accompanied by increased blood flow.. At higher doses of iodide, however, the TSH-induced increases in thyroid hormone release and blood flow are downregulated, and high iodide intake occasionally worsens autoimmune thyroiditis.. To elucidate the genes involved in such effects, we cultured human thyrocytes and examined genes modulated by TSH and iodide, using a cDNA microarray study, which can analyze 2400 genes in each run.

Article Title: Iodide inhibits vascular endothelial growth factor-A expression in cultured human thyroid follicles: a microarray search for effects of thyrotropin and iodide on angiogenesis factors.
Article Snippet: Objective: Excess iodide has been administered to hyperthyroid patients before thyroid surgery to reduce intraoperative bleeding and oozing.. The purpose of this study was to elucidate the mechanism by which iodide reduces blood flow in the hypervascular thyroid gland.. Design: Human thyroid follicles were cultured in the presence or absence of thyrotropin (TSH), or in medium containing various concentrations of iodide, and TSHor iodide-regulated gene expression was analyzed by cDNA microarray.

Article Title: Suppression of iodide uptake and thyroid hormone synthesis with stimulation of the type I interferon system by double-stranded ribonucleic acid in cultured human thyroid follicles.
Article Snippet: .. In brief, RNA was converted to cDNA using a Human cDNA System I Direct Kit (NEN Life Science Products, Boston, MA). cDNA obtained from thyroid follicles cultured in the control and treated media was labeled with Cy3 (green, control) and Cy5 [red, Poly(I:C)], respectively, and the expression levels of 41000 gene spots were analyzed using cDNA microarray (Whole Human Genome Oligo Microarray Kit, Product No. G4112A; Agilent Technologies, Palo Alto, CA). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a nonconfocal laser leader, Gene PX 4000A (Axon Instruments, Union, CA).

Article Title: Genes up- or down-regulated by high calcium medium in parathyroid tissue explants from patients with primary hyperparathyroidism.
Article Snippet: .. after the rNa had been extracted, oligo-dNa microarray was performed as described previously [4]. in brief, rNa was converted to cdNa using a human cdNa system i direct Kit (NeN Life science Products). cdNa obtained from parathyroid tissues cultured in the control and treated media was labeled with Cy3 (green, low calcium) and Cy5 (red, high calcium), respectively, and the expression levels of 41000 gene spots were analyzed using cdNa microarray (Whole human Genome oligo microarray Kit, Product Number G4112a; agilent Technologies, Palo alto, Ca). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a non-confocal laser reader, Gene PX 4000a (axon instruments, union, Ca).

Article Title: Genes up- or down-regulated by high calcium medium in parathyroid tissue explants from patients with primary hyperparathyroidism
Article Snippet: .. In brief, RNA was converted to cDNA using a Human cDNA System I Direct Kit (NEN Life Science Products). cDNA obtained from parathyroid tissues cultured in the control and treated media was labeled with Cy3 (green, low calcium) and Cy5 (red, high calcium), respectively, and the expression levels of 41000 gene spots were analyzed using cDNA microarray (Whole Human Genome Oligo Microarray Kit, Product Number G4112A; Agilent Technologies, Palo Alto, CA). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a non-confocal laser reader, Gene PX 4000A (Axon Instruments, Union, CA).

Control:

Article Title: Stimulating parathyroid cell proliferation and PTH release with phosphate in organ cultures obtained from patients with primary and secondary hyperparathyroidism for a prolonged period.
Article Snippet: The pathogenesis of primary hyperparathyroidism (I -HPT) and secondary hyperparathyroidism (II -HPT) remains to be elucidated.. To characterize their pathophysiology, we investigated the effects of calcium and phosphate on cell proliferation and PTH release in an organ culture of parathyroid tissues.. Dissected parathyroid tissues obtained from patients with I -HPT (adenoma) or II -HPT (nodular hyperplasia) were precultured on a collagen-coated membrane for 1–4 week.

Article Title: Genes regulated by thyrotropin and iodide in cultured human thyroid follicles: analysis by cDNA microarray.
Article Snippet: Thyrotropin (TSH) regulates a number of genes in thyrocytes, leading to iodide uptake, de novo synthesis and release of thyroid hormones, and cell proliferation, accompanied by increased blood flow.. At higher doses of iodide, however, the TSH-induced increases in thyroid hormone release and blood flow are downregulated, and high iodide intake occasionally worsens autoimmune thyroiditis.. To elucidate the genes involved in such effects, we cultured human thyrocytes and examined genes modulated by TSH and iodide, using a cDNA microarray study, which can analyze 2400 genes in each run.

Article Title: Iodide inhibits vascular endothelial growth factor-A expression in cultured human thyroid follicles: a microarray search for effects of thyrotropin and iodide on angiogenesis factors.
Article Snippet: Objective: Excess iodide has been administered to hyperthyroid patients before thyroid surgery to reduce intraoperative bleeding and oozing.. The purpose of this study was to elucidate the mechanism by which iodide reduces blood flow in the hypervascular thyroid gland.. Design: Human thyroid follicles were cultured in the presence or absence of thyrotropin (TSH), or in medium containing various concentrations of iodide, and TSHor iodide-regulated gene expression was analyzed by cDNA microarray.

Article Title: Suppression of iodide uptake and thyroid hormone synthesis with stimulation of the type I interferon system by double-stranded ribonucleic acid in cultured human thyroid follicles.
Article Snippet: .. In brief, RNA was converted to cDNA using a Human cDNA System I Direct Kit (NEN Life Science Products, Boston, MA). cDNA obtained from thyroid follicles cultured in the control and treated media was labeled with Cy3 (green, control) and Cy5 [red, Poly(I:C)], respectively, and the expression levels of 41000 gene spots were analyzed using cDNA microarray (Whole Human Genome Oligo Microarray Kit, Product No. G4112A; Agilent Technologies, Palo Alto, CA). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a nonconfocal laser leader, Gene PX 4000A (Axon Instruments, Union, CA).

Article Title: Genes up- or down-regulated by high calcium medium in parathyroid tissue explants from patients with primary hyperparathyroidism.
Article Snippet: .. after the rNa had been extracted, oligo-dNa microarray was performed as described previously [4]. in brief, rNa was converted to cdNa using a human cdNa system i direct Kit (NeN Life science Products). cdNa obtained from parathyroid tissues cultured in the control and treated media was labeled with Cy3 (green, low calcium) and Cy5 (red, high calcium), respectively, and the expression levels of 41000 gene spots were analyzed using cdNa microarray (Whole human Genome oligo microarray Kit, Product Number G4112a; agilent Technologies, Palo alto, Ca). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a non-confocal laser reader, Gene PX 4000a (axon instruments, union, Ca).

Article Title: Genes up- or down-regulated by high calcium medium in parathyroid tissue explants from patients with primary hyperparathyroidism
Article Snippet: .. In brief, RNA was converted to cDNA using a Human cDNA System I Direct Kit (NEN Life Science Products). cDNA obtained from parathyroid tissues cultured in the control and treated media was labeled with Cy3 (green, low calcium) and Cy5 (red, high calcium), respectively, and the expression levels of 41000 gene spots were analyzed using cDNA microarray (Whole Human Genome Oligo Microarray Kit, Product Number G4112A; Agilent Technologies, Palo Alto, CA). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a non-confocal laser reader, Gene PX 4000A (Axon Instruments, Union, CA).

Labeling:

Article Title: Stimulating parathyroid cell proliferation and PTH release with phosphate in organ cultures obtained from patients with primary and secondary hyperparathyroidism for a prolonged period.
Article Snippet: The pathogenesis of primary hyperparathyroidism (I -HPT) and secondary hyperparathyroidism (II -HPT) remains to be elucidated.. To characterize their pathophysiology, we investigated the effects of calcium and phosphate on cell proliferation and PTH release in an organ culture of parathyroid tissues.. Dissected parathyroid tissues obtained from patients with I -HPT (adenoma) or II -HPT (nodular hyperplasia) were precultured on a collagen-coated membrane for 1–4 week.

Article Title: Genes regulated by thyrotropin and iodide in cultured human thyroid follicles: analysis by cDNA microarray.
Article Snippet: Thyrotropin (TSH) regulates a number of genes in thyrocytes, leading to iodide uptake, de novo synthesis and release of thyroid hormones, and cell proliferation, accompanied by increased blood flow.. At higher doses of iodide, however, the TSH-induced increases in thyroid hormone release and blood flow are downregulated, and high iodide intake occasionally worsens autoimmune thyroiditis.. To elucidate the genes involved in such effects, we cultured human thyrocytes and examined genes modulated by TSH and iodide, using a cDNA microarray study, which can analyze 2400 genes in each run.

Article Title: Iodide inhibits vascular endothelial growth factor-A expression in cultured human thyroid follicles: a microarray search for effects of thyrotropin and iodide on angiogenesis factors.
Article Snippet: Objective: Excess iodide has been administered to hyperthyroid patients before thyroid surgery to reduce intraoperative bleeding and oozing.. The purpose of this study was to elucidate the mechanism by which iodide reduces blood flow in the hypervascular thyroid gland.. Design: Human thyroid follicles were cultured in the presence or absence of thyrotropin (TSH), or in medium containing various concentrations of iodide, and TSHor iodide-regulated gene expression was analyzed by cDNA microarray.

Article Title: Suppression of iodide uptake and thyroid hormone synthesis with stimulation of the type I interferon system by double-stranded ribonucleic acid in cultured human thyroid follicles.
Article Snippet: .. In brief, RNA was converted to cDNA using a Human cDNA System I Direct Kit (NEN Life Science Products, Boston, MA). cDNA obtained from thyroid follicles cultured in the control and treated media was labeled with Cy3 (green, control) and Cy5 [red, Poly(I:C)], respectively, and the expression levels of 41000 gene spots were analyzed using cDNA microarray (Whole Human Genome Oligo Microarray Kit, Product No. G4112A; Agilent Technologies, Palo Alto, CA). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a nonconfocal laser leader, Gene PX 4000A (Axon Instruments, Union, CA).

Article Title: Genes up- or down-regulated by high calcium medium in parathyroid tissue explants from patients with primary hyperparathyroidism.
Article Snippet: .. after the rNa had been extracted, oligo-dNa microarray was performed as described previously [4]. in brief, rNa was converted to cdNa using a human cdNa system i direct Kit (NeN Life science Products). cdNa obtained from parathyroid tissues cultured in the control and treated media was labeled with Cy3 (green, low calcium) and Cy5 (red, high calcium), respectively, and the expression levels of 41000 gene spots were analyzed using cdNa microarray (Whole human Genome oligo microarray Kit, Product Number G4112a; agilent Technologies, Palo alto, Ca). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a non-confocal laser reader, Gene PX 4000a (axon instruments, union, Ca).

Article Title: Genes up- or down-regulated by high calcium medium in parathyroid tissue explants from patients with primary hyperparathyroidism
Article Snippet: .. In brief, RNA was converted to cDNA using a Human cDNA System I Direct Kit (NEN Life Science Products). cDNA obtained from parathyroid tissues cultured in the control and treated media was labeled with Cy3 (green, low calcium) and Cy5 (red, high calcium), respectively, and the expression levels of 41000 gene spots were analyzed using cDNA microarray (Whole Human Genome Oligo Microarray Kit, Product Number G4112A; Agilent Technologies, Palo Alto, CA). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a non-confocal laser reader, Gene PX 4000A (Axon Instruments, Union, CA).

Expressing:

Article Title: Stimulating parathyroid cell proliferation and PTH release with phosphate in organ cultures obtained from patients with primary and secondary hyperparathyroidism for a prolonged period.
Article Snippet: The pathogenesis of primary hyperparathyroidism (I -HPT) and secondary hyperparathyroidism (II -HPT) remains to be elucidated.. To characterize their pathophysiology, we investigated the effects of calcium and phosphate on cell proliferation and PTH release in an organ culture of parathyroid tissues.. Dissected parathyroid tissues obtained from patients with I -HPT (adenoma) or II -HPT (nodular hyperplasia) were precultured on a collagen-coated membrane for 1–4 week.

Article Title: Genes regulated by thyrotropin and iodide in cultured human thyroid follicles: analysis by cDNA microarray.
Article Snippet: Thyrotropin (TSH) regulates a number of genes in thyrocytes, leading to iodide uptake, de novo synthesis and release of thyroid hormones, and cell proliferation, accompanied by increased blood flow.. At higher doses of iodide, however, the TSH-induced increases in thyroid hormone release and blood flow are downregulated, and high iodide intake occasionally worsens autoimmune thyroiditis.. To elucidate the genes involved in such effects, we cultured human thyrocytes and examined genes modulated by TSH and iodide, using a cDNA microarray study, which can analyze 2400 genes in each run.

Article Title: Iodide inhibits vascular endothelial growth factor-A expression in cultured human thyroid follicles: a microarray search for effects of thyrotropin and iodide on angiogenesis factors.
Article Snippet: Objective: Excess iodide has been administered to hyperthyroid patients before thyroid surgery to reduce intraoperative bleeding and oozing.. The purpose of this study was to elucidate the mechanism by which iodide reduces blood flow in the hypervascular thyroid gland.. Design: Human thyroid follicles were cultured in the presence or absence of thyrotropin (TSH), or in medium containing various concentrations of iodide, and TSHor iodide-regulated gene expression was analyzed by cDNA microarray.

Article Title: Suppression of iodide uptake and thyroid hormone synthesis with stimulation of the type I interferon system by double-stranded ribonucleic acid in cultured human thyroid follicles.
Article Snippet: .. In brief, RNA was converted to cDNA using a Human cDNA System I Direct Kit (NEN Life Science Products, Boston, MA). cDNA obtained from thyroid follicles cultured in the control and treated media was labeled with Cy3 (green, control) and Cy5 [red, Poly(I:C)], respectively, and the expression levels of 41000 gene spots were analyzed using cDNA microarray (Whole Human Genome Oligo Microarray Kit, Product No. G4112A; Agilent Technologies, Palo Alto, CA). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a nonconfocal laser leader, Gene PX 4000A (Axon Instruments, Union, CA).

Article Title: Genes up- or down-regulated by high calcium medium in parathyroid tissue explants from patients with primary hyperparathyroidism.
Article Snippet: .. after the rNa had been extracted, oligo-dNa microarray was performed as described previously [4]. in brief, rNa was converted to cdNa using a human cdNa system i direct Kit (NeN Life science Products). cdNa obtained from parathyroid tissues cultured in the control and treated media was labeled with Cy3 (green, low calcium) and Cy5 (red, high calcium), respectively, and the expression levels of 41000 gene spots were analyzed using cdNa microarray (Whole human Genome oligo microarray Kit, Product Number G4112a; agilent Technologies, Palo alto, Ca). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a non-confocal laser reader, Gene PX 4000a (axon instruments, union, Ca).

Article Title: Genes up- or down-regulated by high calcium medium in parathyroid tissue explants from patients with primary hyperparathyroidism
Article Snippet: .. In brief, RNA was converted to cDNA using a Human cDNA System I Direct Kit (NEN Life Science Products). cDNA obtained from parathyroid tissues cultured in the control and treated media was labeled with Cy3 (green, low calcium) and Cy5 (red, high calcium), respectively, and the expression levels of 41000 gene spots were analyzed using cDNA microarray (Whole Human Genome Oligo Microarray Kit, Product Number G4112A; Agilent Technologies, Palo Alto, CA). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a non-confocal laser reader, Gene PX 4000A (Axon Instruments, Union, CA).

Microarray:

Article Title: Stimulating parathyroid cell proliferation and PTH release with phosphate in organ cultures obtained from patients with primary and secondary hyperparathyroidism for a prolonged period.
Article Snippet: The pathogenesis of primary hyperparathyroidism (I -HPT) and secondary hyperparathyroidism (II -HPT) remains to be elucidated.. To characterize their pathophysiology, we investigated the effects of calcium and phosphate on cell proliferation and PTH release in an organ culture of parathyroid tissues.. Dissected parathyroid tissues obtained from patients with I -HPT (adenoma) or II -HPT (nodular hyperplasia) were precultured on a collagen-coated membrane for 1–4 week.

Article Title: Genes regulated by thyrotropin and iodide in cultured human thyroid follicles: analysis by cDNA microarray.
Article Snippet: Thyrotropin (TSH) regulates a number of genes in thyrocytes, leading to iodide uptake, de novo synthesis and release of thyroid hormones, and cell proliferation, accompanied by increased blood flow.. At higher doses of iodide, however, the TSH-induced increases in thyroid hormone release and blood flow are downregulated, and high iodide intake occasionally worsens autoimmune thyroiditis.. To elucidate the genes involved in such effects, we cultured human thyrocytes and examined genes modulated by TSH and iodide, using a cDNA microarray study, which can analyze 2400 genes in each run.

Article Title: Iodide inhibits vascular endothelial growth factor-A expression in cultured human thyroid follicles: a microarray search for effects of thyrotropin and iodide on angiogenesis factors.
Article Snippet: Objective: Excess iodide has been administered to hyperthyroid patients before thyroid surgery to reduce intraoperative bleeding and oozing.. The purpose of this study was to elucidate the mechanism by which iodide reduces blood flow in the hypervascular thyroid gland.. Design: Human thyroid follicles were cultured in the presence or absence of thyrotropin (TSH), or in medium containing various concentrations of iodide, and TSHor iodide-regulated gene expression was analyzed by cDNA microarray.

Article Title: Suppression of iodide uptake and thyroid hormone synthesis with stimulation of the type I interferon system by double-stranded ribonucleic acid in cultured human thyroid follicles.
Article Snippet: .. In brief, RNA was converted to cDNA using a Human cDNA System I Direct Kit (NEN Life Science Products, Boston, MA). cDNA obtained from thyroid follicles cultured in the control and treated media was labeled with Cy3 (green, control) and Cy5 [red, Poly(I:C)], respectively, and the expression levels of 41000 gene spots were analyzed using cDNA microarray (Whole Human Genome Oligo Microarray Kit, Product No. G4112A; Agilent Technologies, Palo Alto, CA). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a nonconfocal laser leader, Gene PX 4000A (Axon Instruments, Union, CA).

Article Title: Genes up- or down-regulated by high calcium medium in parathyroid tissue explants from patients with primary hyperparathyroidism.
Article Snippet: .. after the rNa had been extracted, oligo-dNa microarray was performed as described previously [4]. in brief, rNa was converted to cdNa using a human cdNa system i direct Kit (NeN Life science Products). cdNa obtained from parathyroid tissues cultured in the control and treated media was labeled with Cy3 (green, low calcium) and Cy5 (red, high calcium), respectively, and the expression levels of 41000 gene spots were analyzed using cdNa microarray (Whole human Genome oligo microarray Kit, Product Number G4112a; agilent Technologies, Palo alto, Ca). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a non-confocal laser reader, Gene PX 4000a (axon instruments, union, Ca).

Article Title: Genes up- or down-regulated by high calcium medium in parathyroid tissue explants from patients with primary hyperparathyroidism
Article Snippet: .. In brief, RNA was converted to cDNA using a Human cDNA System I Direct Kit (NEN Life Science Products). cDNA obtained from parathyroid tissues cultured in the control and treated media was labeled with Cy3 (green, low calcium) and Cy5 (red, high calcium), respectively, and the expression levels of 41000 gene spots were analyzed using cDNA microarray (Whole Human Genome Oligo Microarray Kit, Product Number G4112A; Agilent Technologies, Palo Alto, CA). .. Laser detection of the Cy3 and Cy5 signals on the microarray was performed with a non-confocal laser reader, Gene PX 4000A (Axon Instruments, Union, CA).



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